Translational_Unit

Part:BBa_K1587002

Designed by: Alexandre Le Scornet   Group: iGEM15_Toulouse   (2015-09-13)

pcyA with RBS

Strong RBS (BBa_B0030). Ferredoxin oxidoreductase (PcyA) from synechocystis (BBa_I15009). Converts biliverdin IXalpha (BV) to phycocyanobilin (PCB), the immediate precursor of cyanobacterial phytochromes (Figure 1).
The integrity of our construct was checked by sequencing using VF2 primer.

TLSE pcyA ho1.jpg

Figure 1: Phycobilin biosynthetic pathway in cyanobacteria showing the formation of PCB from heme. The first step comprises HOI-catalyzed heme degradation. (Ken et al 2009)

pcyA is used to catalyse the formation of phycocyanobilin (PCB), a chromophore of the phytochrome family of photoreceptors, photosynthetic light-harvesting complexes required for circadian clock. It is usually used in association with ho1 that catalyses the formation of biliverdin (BV) IXα via heme.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 790
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 343
  • 1000
    COMPATIBLE WITH RFC[1000]


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Categories
Parameters
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